Journal: Cell Communication and Signaling : CCS
Article Title: L-Fucose promotes enteric nervous system regeneration in type 1 diabetic mice by inhibiting SMAD2 signaling pathway in enteric neural precursor cells
doi: 10.1186/s12964-023-01311-0
Figure Lengend Snippet: L-Fucose improved the proliferation and differentiation abilities of injured ENPCs in vitro. A Cell morphology of primary ENPCs at days 2, 4, and 7. B Immunofluorescence staining showed cells within neurospheres co-expressed Ngfr (green) and Nestin (red), but immuno-negative for glial cell marker GFAP (green) or neuronal marker PGP9.5 (red). By inducing differentiation, neurons (PGP9.5, red), glial cells (GFAP, green), and smooth muscle cells (α-SMA, gray) were derived from neurospheres; the nuclei (blue). C Cell viability of ENPCs treated with different concentrations of L-Fucose for 72 h was determined by CCK8 assay. D Ki67 staining of ENPCs in each group. E The positive rate of Ki67 was analyzed in the statistical chart. F Representative immunofluorescence images of ENPCs-derived neurons (HuC/D, green) or glial cells (GFAP, green) in each group; the nuclei (blue). G The positive rate of HuC/D and GFAP were analyzed in the statistical chart. H Western blot analysis of HuC/D and GFAP proteins in each group. I Densitometric analysis of HuC/D and GFAP proteins in each group. Con: Primary ENPCs cultures; Glu: ENPCs treated with 30 mM glucose; Fuc: ENPCs treated with 30 mM glucose and 30 mg/ml L-Fucose. ENPCs, enteric neural precursor cells. Results were expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Article Snippet: Nestin-creER T2 mice were crossed with R26-e(CAG-RSR-LSL-DTRGFP-WPRE-pA) and Ngfr-e(2 A-DreER T2 ) mice (purchased from Shanghai Model Organisms Center, Inc) to generate Nestin-creER T2 × Ngfr-DreER T2 : DTRGFP triple transgenic mice, which specially labeled newborn Nestin + /Ngfr + cells with green fluorescent protein (GFP) after tamoxifen (TAM) induction (Additional file : Fig. S1A).
Techniques: In Vitro, Immunofluorescence, Staining, Marker, Derivative Assay, CCK-8 Assay, Western Blot, Standard Deviation